![Figure 1 from Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. | Semantic Scholar Figure 1 from Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/e45dac9600878bc674dd3f3479a340c447442f4c/3-Figure1-1.png)
Figure 1 from Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. | Semantic Scholar
![The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins | Nature Protocols The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins | Nature Protocols](https://media.springernature.com/m685/springer-static/image/art%3A10.1038%2Fnprot.2007.209/MediaObjects/41596_2007_Article_BFnprot2007209_Fig1_HTML.jpg)
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins | Nature Protocols
![Figure 2 from Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. | Semantic Scholar Figure 2 from Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. | Semantic Scholar](https://d3i71xaburhd42.cloudfront.net/e45dac9600878bc674dd3f3479a340c447442f4c/4-Figure2-1.png)
Figure 2 from Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants. | Semantic Scholar
![One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3) | Protocol (Translated to Portuguese) One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3) | Protocol (Translated to Portuguese)](https://cloudfront.jove.com/files/ftp_upload/51536/51536fig1highres.jpg)
One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3) | Protocol (Translated to Portuguese)
![IBA Lifesciences on X: "❓After elution from the column, are Strep-tag®II or Twin-Strep-tag® proteins in complex with (desthio-)biotin? No, (desthio-)biotin doesn't bind to the target protein. After elution, (desthio-)biotin can be removed IBA Lifesciences on X: "❓After elution from the column, are Strep-tag®II or Twin-Strep-tag® proteins in complex with (desthio-)biotin? No, (desthio-)biotin doesn't bind to the target protein. After elution, (desthio-)biotin can be removed](https://pbs.twimg.com/media/FBuUoiSX0BE6ZM3.jpg)